The Determination of Acetone and Acetoacetic Acid in Blood by the Bisulfite-binding Method and Its Relation to Pyruvic Acid
نویسنده
چکیده
Clift and Cook (1) have described a method for the determination of carbonyl compounds in blood as bisulfite-binding substances. Devised primarily for rapid determination of pyruvic acid, the procedure as described made possible the determination of acetoacetic acid to the extent of about 87 per cent, but acetonc could not be measured because of the instability of the addition product. Sufficient research has been carried out by a number of workers to establish the proper conditions for the quantitative recovery of acetone in pure solutions as well as acetoacetic acid by the bisulfite addition reaction. Equilibrium studies with carbonyl-bisulfite compounds by Kerp and his collaborators are reported in some detail by Kolthoff et al. (2) and Parkinson and Wagner (3). Jolles (4) and Stepp and Engelhardt (5) also describe a somewhat unwieldy method for the estimation of large amounts of acetone as bisulfite-binding substances. Modifications of the Clift and Cook method have been reported (6-9), but nom provides the conditions for completely stabilizing the acetoncbisulfite compound. A distinct need exists for a simple method for the determination of ketone substances. By introducing modifications into the Clift and Cook procedure it has been possible to adapt it to t’hc determination of acetone and acetoacetic acid in blood. Reagents~/12 sulfuric acid solution. 10 per cent sodium tungstate solution. 25 per cent copper sulfate solution.
منابع مشابه
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تاریخ انتشار 2003